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The trial enrolled 300 obese subjects (BMI 27-35 kg/m) across multiple centers and randomized them to AOD-9604 (3 mg subcutaneous injection once or twice daily) or placebo for 12 weeks

Animals were trained for 3 consecutive days prior to MCAO, with three trials per day

Many peptides produce gradual improvements over weeks to months as they optimize natural biological pathways

Compared to the DSS-treated group, flaxseed oil treatment for six weeks raised SOD activity and GSH levels while decreasing MDA levels and MPO activity (127) ( Supplementary Table 1 )

Adults of 2123 days old were also used in the gene expression assay (next paragraph)